Between 2021-2025, the I2I Methods Validation Team critically reviewed bioassays and related methods used in the development and evaluation of vector control tools to identify and minimise sources of variability and identify gaps in our knowledge of how best to perform testing for existing and new tools. The team addressed these gaps by developing consensus protocols, experimentally validating existing and new methods, and engaging with stakeholders to identify concerns and generate solutions, including best practice protocols and data analysis tools.