Standard Operating Procedures

I2I has collated a number of ‘best practice’ SOPs for the laboratory and semi-field testing of LLIN and IRS products from global testing sites. The SOPs have been reviewed by a panel of independent experts and are deliberately un-formatted for easy incorporation into site specific SOP files and adapted to location specific contexts.

12th August 2022

SOP: Spraying with the potter tower

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12th August 2022

SOP: IRS Block Preparation

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11th August 2022

SOP: Cone bioassay of treated blocks

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18th January 2023

SOP: Transportation of mosquitoes

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18th January 2023

SOP: Scoring mosquitoes after hut IRS

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18th January 2023

SOP: Removal of ant infestations from experimental huts

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4th January 2023

SOP: Preparation of sugar-soaked cotton wool for feeding adult mosquitoes

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12th August 2022

SOP: Preparation of experimental huts for evaluation of IRS

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12th August 2022

SOP: Indoor residual spraying of experimental huts for evaluation of insecticides

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12th August 2022

SOP: Cone bioassay on IRS treated surfaces in experimental huts

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17th August 2023

SOP: WHO wire ball bioassay

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17th August 2023

SOP: Extraction of active ingredient (AI) from a treated filter paper

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17th August 2023

SOP: HPLC quantification of surface and total active ingredient present on an ITN

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17th August 2023

SOP: Net in tube bioassay – pyrethroid-chlorfenapyr nets

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17th August 2023

SOP: Dissection and measurement of mosquito wings

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18th July 2023

SOP: Intrinsic Toxicity

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18th July 2023

SOP: Wind Tunnel Testing

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10th May 2023

NIRI SOP: Assessment of Abrasion Resistance of Insecticide-Treated Nets (ITNs)

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10th May 2023

NIRI SOP: Assessment of Hole Enlargement Resistance in Insecticide-Treated Nets (ITNs)

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10th May 2023

NIRI SOP: Snag Strength Test for Insecticide-Treated Nets (ITNs)

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14th July 2022

SOP: Methods for monitoring the durability of dual-AI insecticide-treated nets containing a pyrethroid plus pyriproxyfen (PPF)

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14th July 2022

SOP: Methods for monitoring the durability of dual-AI insecticide-treated nets containing a pyrethroid plus piperonyl butoxide (PBO)

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14th July 2022

SOP: Methods for monitoring the durability of dual-AI insecticide-treated nets containing a pyrethroid plus chlorfenapyr (CFP)

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14th July 2022

SOP: Strain characterisation of resistant mosquitoes for monitoring bioefficacy in ITNs treated with two active ingredients (Dual-AI ITNs)

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7th July 2022

SOP: Net washing for laboratory LLIN trials

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7th July 2022

SOP: Cutting and preserving net samples for chemical analysis

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7th July 2022

SOP: Tunnel test

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7th July 2022

SOP: Cone bioassay

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25th October 2023

SOP: Experimental hut preparation and running

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17th August 2023

SOP: Ifakara Ambient Chamber Test (I-ACT)

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17th August 2023

SOP: Dissection and measurement of mosquito wings

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18th January 2023

SOP: Transportation of mosquitoes

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18th January 2023

SOP: Removal of ant infestations from experimental huts

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4th January 2023

SOP: Preparation of sugar-soaked cotton wool for feeding adult mosquitoes

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4th January 2023

SOP: Mosquito collections from experimental huts

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7th July 2022

SOP: Net washing for semifield trials

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18th July 2023

SOP: Insect Growth Regulator Larvicides

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18th July 2023

SOP: Bacterial Larvicide Bioassay

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18th July 2023

SOP: Field Evaluation of Microbial Mosquito Larvicide Efficacy

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18th July 2023

SOP: Intrinsic Toxicity

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18th July 2023

SOP: Semi-Field Trials: Open Field for Aerial Application

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18th July 2023

SOP: Semi-Field Trials: Open Field for Ground Application

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18th July 2023

SOP: Calibration for Aerial Application Equipment

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18th July 2023

SOP: Calibration for Backpack and Truck-Mounted Sprayers

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18th July 2023

SOP: Operational Field Trials: Ground and Aerial

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