Standard Operating Procedures

I2I has collated a number of ‘best practice’ SOPs for the laboratory and semi-field testing of LLIN and IRS products from global testing sites. The SOPs have been reviewed by a panel of independent experts and are deliberately un-formatted for easy incorporation into site specific SOP files and adapted to location specific contexts.

A hand wearing a glove holding two test tubes holding mosquitoes horizontally across the image and a net in the background where mosquitoes are resting

12th August 2022

SOP: Spraying with the potter tower

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Mosquito cages used during IRS campaign in Ethiopia. Mosquito cage on right side of image and a woman in jeans and a sweatshirt on the right side of the image inserting her arm into the cage.

12th August 2022

SOP: IRS Block Preparation

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IRS sprayer sitting in a green chair wearing protective equipment and speaking with a mother and child outside of a rural home with a thatched roof

11th August 2022

SOP: Cone bioassay of treated blocks

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Bird's eye view of numerous mosquitoes inside of paper cup covered with transparent netting with a white cotton ball partially inserted into the netting

18th January 2023

SOP: Transportation of mosquitoes

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18th January 2023

SOP: Scoring mosquitoes after hut IRS

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Dirt road leading to huts within green foliage

18th January 2023

SOP: Removal of ant infestations from experimental huts

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Entomological sample storage

4th January 2023

SOP: Preparation of sugar-soaked cotton wool for feeding adult mosquitoes

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IRS spraying equipment in the corner of a green room with a brown floor and a box with equipment to the right of the image.

12th August 2022

SOP: Preparation of experimental huts for evaluation of IRS

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Spray campaign in Zimbabwe showing a rural home with a thatched roof on the left and a spray operator in yellow PPE and a woman with a purple head scarf and a man with a baseball cap on the right side and trees in the background and blue sky behind.

12th August 2022

SOP: Indoor residual spraying of experimental huts for evaluation of insecticides

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IRS spray operator outside house with family during IRS campaign, Ethiopia, 2014

12th August 2022

SOP: Cone bioassay on IRS treated surfaces in experimental huts

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Silver metal circle with red sandpaper at the centre of the circle.

10th May 2023

NIRI SOP: Assessment of Abrasion Resistance of Insecticide-Treated Nets (ITNs)

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Image of blue netting against a black background with a hole in the bottom centre of the net fabric. A silver ruler at the bottom of the image is laid across the diameter of the hole, showing the hole is 3 cm wide.

10th May 2023

NIRI SOP: Assessment of Hole Enlargement Resistance in Insecticide-Treated Nets (ITNs)

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Diagram from Figure 1 of Snag Strength NIRI SOP showing a diagonal image of 6 squares with six black loops within each square. An image of a needle is in each of the three left, top squares, each attached to the three loops. A diagonal blue arrow runs from the bottom left of the diagonal grid with the label Wales. A blue arrow runs from top to bottom of the grid labelled Bias. An arrow runs from the bottom right of the grid labelled Courses.

10th May 2023

NIRI SOP: Snag Strength Test for Insecticide-Treated Nets (ITNs)

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Piece of white mosquito net with dark beige background

14th July 2022

SOP: Methods for monitoring the durability of dual-AI insecticide-treated nets containing a pyrethroid plus pyriproxyfen (PPF)

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A piece of mosquito net on a beige background

14th July 2022

SOP: Methods for monitoring the durability of dual-AI insecticide-treated nets containing a pyrethroid plus piperonyl butoxide (PBO)

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Picture of net fabric

14th July 2022

SOP: Methods for monitoring the durability of dual-AI insecticide-treated nets containing a pyrethroid plus chlorfenapyr (CFP)

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Anopheles gambiae mosquito feeding on a human finger with bright blue background. Mosquito is diagonal and front facing to the camera.

14th July 2022

SOP: Strain characterisation of resistant mosquitoes for monitoring bioefficacy in ITNs treated with two active ingredients (Dual-AI ITNs)

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Distributing long lasting insecticide-treated nets (LLINs) in Ethiopia

7th July 2022

SOP: Net washing for laboratory LLIN trials

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Close up picture of bed net fibers

7th July 2022

SOP: Cutting and preserving net samples for chemical analysis

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Mosquito cage showing a black metal frame and netting on all sides with a knot in the netting in the front to close the opening. Numerous mosquitoes are inside.

7th July 2022

SOP: Tunnel test

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Five mosquitoes inside a paper cup covered in netting for testing

7th July 2022

SOP: Cone bioassay

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Close up picture of bed net fibers

30th October 2020

Archived SOP: Cutting and preserving net samples for chemical analysis

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Bird's eye view of numerous mosquitoes inside of paper cup covered with transparent netting with a white cotton ball partially inserted into the netting

18th January 2023

SOP: Transportation of mosquitoes

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Dirt road leading to huts within green foliage

18th January 2023

SOP: Removal of ant infestations from experimental huts

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Entomological sample storage

4th January 2023

SOP: Preparation of sugar-soaked cotton wool for feeding adult mosquitoes

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November 2018 spray campaign in Zimbabwe

4th January 2023

SOP: Mosquito collections from experimental huts

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Net Distribution In Mwanza, Tanzania 2016

14th July 2022

SOP: Experimental hut preparation and running

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Net Distribution In Mwanza, Tanzania 2016

7th July 2022

SOP: Net washing for semifield trials

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Mosquito larvae

10th August 2021

Insect Growth Regulator Larvicides

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Larvae breeding at Liberia national malaria control program (NMCP) insectary.

13th July 2021

Bacterial Larvicide Bioassay

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Laboratory scientist holds a container of mosquito pupa in the new insectary at Africa University in Mutare, Zimbabwe. Photo by Bridget Higginbotham for USAID/PMI, 2018.

10th August 2021

Field Evaluation of Microbial Mosquito Larvicide Efficacy

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Wind tunnel configuration, showing the twin fluid gravity fed venturi nozzle, the baffled opening for air straightening, opening downwind from the point of atomization and at the rear an extraction fan.

10th August 2021

Intrinsic Toxicity and Wind Tunnel Testing

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Dirt road leading to huts within green foliage

10th August 2021

Semi-Field Trials: Open Field for Ground Application

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Sun shining down on a building in a rural landscape with trees in Zimbabwe

10th August 2021

Semi-Field Trials: Open Field for Aerial Application

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Image of hands using a pen to complete a form

10th August 2021

Calibration for Backpack and Truck-Mounted Sprayers

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Ugandan landscape showing a dirt road, trees, house and mountain in the distance

10th August 2021

Operational Field Trials: Ground and Aerial

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