Developing Consensus Standard Operating Procedures (SOPs) to Evaluate New Types of Insecticide-Treated Nets |

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Developing Consensus Standard Operating Procedures (SOPs) to Evaluate New Types of Insecticide-Treated Nets

Infographic showing a red arrow pointing from left to right with four circles increasing in size from left to right representing four milestones in the process of method development used for producing consensus SOPs for biological durability monitoring of new net types

Experts have published updated protocols for assessing the durability of insecticide-treated bednets containing second active ingredients to fight insecticide resistance.

Following a recent consultation of relevant stakeholders, of university researchers, research organisations involved in ITNs durability monitoring studies, and procurement bodies, a new proposed set of guidelines and SOPs for assessing the durability of several dual-AI ITNs was agreed. The work is now published in the special issue on insecticides for mosquito control in the journal Insects. The publication focuses on the methods used to develop this set of optimized SOPs, which assess the bioefficacy of nets containing a pyrethroid plus either chlorfenapyr, PBO or PPF. The methods and SOPs have been discussed and agreed with all the relevant stakeholders.

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SOPs

Insects, Special Issue: Insecticides for Mosquito Control

White lab bench diagonal from left to right across the image with two long rows of glass bottles with black lids in the forefront of the image and paper cups with netting in the back with a blue lab notebook

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Insecticides for Mosquito Control: Improving and Validating Methods to Strengthen the Evidence Base

Image of an estuary with a grey and black drone hovering above the right side of the image with a blue sky in the background

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Unmanned aerial vehicles for surveillance and control of vectors of malaria and other vector-borne diseases

Three, uncapped, clear bottles placed horizontally on a blue and white roller with black knobs with the top end of the bottles facing the left of the image near the black knobs.

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A new WHO bottle bioassay method to assess the susceptibility of mosquito vectors to public health insecticides

Image of 8 circles in a linear zig-zag shape to demonstrate the possible approaches to achieve sufficient standardisation or characterisation of pyrethroid-resistant mosquitoes used for bioefficacy bioassays of dual-AI ITNs to compile, compare, and interpret results across studies

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Strain Characterisation for Measuring Bioefficacy of ITNs Treated with Two Active Ingredients (Dual-AI ITNs)

An image of 6 circles increasing in size, length and proportion of dark to white colouration from left to right, categorised into five numerical categories with the first five circles labelled infertile. The image represents Christopher stages of egg development. Mosquitos whose eggs have fully developed to stage V (normal elongated, boat/sausage-shaped eggs with lateral floats) are classified as ‘fecund’ or ‘fertile’. If eggs have not fully developed and remain in stages I–IV (less elongated, round shape, lacking floats), the mosquito is classified as ‘non-fecund’ or ‘infertile’.

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