Standard Operating Procedures

I2I has collated a number of ‘best practice’ SOPs for the laboratory and semi-field testing of LLIN and IRS products from global testing sites. The SOPs have been reviewed by a panel of independent experts and are deliberately un-formatted for easy incorporation into site specific SOP files and adapted to location specific contexts.

5th April 2024

SOP: Methods for Performing Calculations and Dilutions

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A hand wearing a glove holding two test tubes holding mosquitoes horizontally across the image and a net in the background where mosquitoes are resting

12th August 2022

SOP: Spraying with the potter tower

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Mosquito cages used during IRS campaign in Ethiopia. Mosquito cage on right side of image and a woman in jeans and a sweatshirt on the right side of the image inserting her arm into the cage.

12th August 2022

SOP: IRS Block Preparation

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IRS sprayer sitting in a green chair wearing protective equipment and speaking with a mother and child outside of a rural home with a thatched roof

11th August 2022

SOP: Cone bioassay of treated blocks

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Bird's eye view of numerous mosquitoes inside of paper cup covered with transparent netting with a white cotton ball partially inserted into the netting

18th January 2023

SOP: Transportation of mosquitoes

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18th January 2023

SOP: Scoring mosquitoes after hut IRS

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Dirt road leading to huts within green foliage

18th January 2023

SOP: Removal of ant infestations from experimental huts

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Entomological sample storage

4th January 2023

SOP: Preparation of sugar-soaked cotton wool for feeding adult mosquitoes

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IRS spraying equipment in the corner of a green room with a brown floor and a box with equipment to the right of the image.

12th August 2022

SOP: Preparation of experimental huts for evaluation of IRS

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Spray campaign in Zimbabwe showing a rural home with a thatched roof on the left and a spray operator in yellow PPE and a woman with a purple head scarf and a man with a baseball cap on the right side and trees in the background and blue sky behind.

12th August 2022

SOP: Indoor residual spraying of experimental huts for evaluation of insecticides

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IRS spray operator outside house with family during IRS campaign, Ethiopia, 2014

12th August 2022

SOP: Cone bioassay on IRS treated surfaces in experimental huts

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Mosquito cage showing a black metal frame and netting on all sides with a knot in the netting in the front to close the opening. Numerous mosquitoes are inside.

13th August 2024

SOP: Tunnel Test

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25 small rectangles of blue net material hung side over white line over three rows. Each is held in place by a small wooden clip and labelled with a small rectangle of white paper.

13th August 2024

SOP: Net washing for laboratory LLIN trials

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Image of the transparent cone of a cone bioassay against a white board with the narrow opening of the cone in the upper right quadrant plugged with a cotton ball. Five mosquitoes rest on the inside of the walls of the cone.

7th August 2024

SOP: Cone Bioassay

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5th April 2024

SOP: Methods for Performing Calculations and Dilutions

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Silver, metal frame cage covered in white netting and secured with rubber bands at the corners with mosquitoes inside

17th August 2023

SOP: WHO wire ball bioassay

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Open blue pipette tip box in upper left corner of image with dark blue and white pipette without tip lying on a white lab table

17th August 2023

SOP: Extraction of active ingredient (AI) from a treated filter paper

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Blue bed net material with beige wall behind

17th August 2023

SOP: HPLC quantification of surface and total active ingredient present on an ITN

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Downward view of mosquitoes resting on the inside of a clear WHO test tube.

17th August 2023

SOP: Net in tube bioassay – pyrethroid-chlorfenapyr nets

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Sepia coloured microscopy image of two mosquito wings showing alternating dark and light pattern across the top of the wings, and fibers along the bottom edge of the wings against a ruler

17th August 2023

SOP: Dissection and measurement of mosquito wings

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18th July 2023

SOP: Intrinsic Toxicity

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Wind tunnel configuration, showing the twin fluid gravity fed venturi nozzle, the baffled opening for air straightening, opening downwind from the point of atomization and at the rear an extraction fan.

18th July 2023

SOP: Wind Tunnel Testing

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Silver metal circle with red sandpaper at the centre of the circle.

10th May 2023

NIRI SOP: Assessment of Abrasion Resistance of Insecticide-Treated Nets (ITNs)

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Image of blue netting against a black background with a hole in the bottom centre of the net fabric. A silver ruler at the bottom of the image is laid across the diameter of the hole, showing the hole is 3 cm wide.

10th May 2023

NIRI SOP: Assessment of Hole Enlargement Resistance in Insecticide-Treated Nets (ITNs)

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Diagram from Figure 1 of Snag Strength NIRI SOP showing a diagonal image of 6 squares with six black loops within each square. An image of a needle is in each of the three left, top squares, each attached to the three loops. A diagonal blue arrow runs from the bottom left of the diagonal grid with the label Wales. A blue arrow runs from top to bottom of the grid labelled Bias. An arrow runs from the bottom right of the grid labelled Courses.

10th May 2023

NIRI SOP: Snag Strength Test for Insecticide-Treated Nets (ITNs)

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Piece of white mosquito net with dark beige background

14th July 2022

SOP: Methods for monitoring the durability of dual-AI insecticide-treated nets containing a pyrethroid plus pyriproxyfen (PPF)

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A piece of mosquito net on a beige background

14th July 2022

SOP: Methods for monitoring the durability of dual-AI insecticide-treated nets containing a pyrethroid plus piperonyl butoxide (PBO)

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Picture of net fabric

14th July 2022

SOP: Methods for monitoring the durability of dual-AI insecticide-treated nets containing a pyrethroid plus chlorfenapyr (CFP)

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Anopheles gambiae mosquito feeding on a human finger with bright blue background. Mosquito is diagonal and front facing to the camera.

14th July 2022

SOP: Strain characterisation of resistant mosquitoes for monitoring bioefficacy in ITNs treated with two active ingredients (Dual-AI ITNs)

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Close up picture of bed net fibers

7th July 2022

SOP: Cutting and preserving net samples for chemical analysis

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Woman holding a rice bowl and wearing a pink hat and grey shirt sitting in house with blue bednet behind her.

25th October 2023

SOP: Experimental hut preparation and running

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Three mosquitoes resting on the wall of a mesh fabric

17th August 2023

SOP: Ifakara Ambient Chamber Test (I-ACT)

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Sepia coloured microscopy image of two mosquito wings showing alternating dark and light pattern across the top of the wings, and fibers along the bottom edge of the wings against a ruler

17th August 2023

SOP: Dissection and measurement of mosquito wings

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Bird's eye view of numerous mosquitoes inside of paper cup covered with transparent netting with a white cotton ball partially inserted into the netting

18th January 2023

SOP: Transportation of mosquitoes

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Dirt road leading to huts within green foliage

18th January 2023

SOP: Removal of ant infestations from experimental huts

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Entomological sample storage

4th January 2023

SOP: Preparation of sugar-soaked cotton wool for feeding adult mosquitoes

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November 2018 spray campaign in Zimbabwe

4th January 2023

SOP: Mosquito collections from experimental huts

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Net Distribution In Mwanza, Tanzania 2016

7th July 2022

SOP: Net washing for semifield trials

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Laboratory scientist holds a container of mosquito pupa in the new insectary at Africa University in Mutare, Zimbabwe. Photo by Bridget Higginbotham for USAID/PMI, 2018.

5th April 2024

SOP: Performing Larval Insecticide Bioassays and Larval Susceptibility Testing

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Mosquito larvae

18th July 2023

SOP: Insect Growth Regulator Larvicides

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Larvae breeding at Liberia national malaria control program (NMCP) insectary.

18th July 2023

SOP: Bacterial Larvicide Bioassay

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Community Health Volunteers collecting larvae, Liberia, 2018

18th July 2023

SOP: Field Evaluation of Microbial Mosquito Larvicide Efficacy

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18th July 2023

SOP: Intrinsic Toxicity

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Sun shining down on a building in a rural landscape with trees in Zimbabwe

18th July 2023

SOP: Semi-Field Trials: Open Field for Aerial Application

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Dirt road leading to huts within green foliage

18th July 2023

SOP: Semi-Field Trials: Open Field for Ground Application

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Picture of a hand entering numbers into a calculator while holding a document

18th July 2023

SOP: Calibration for Aerial Application Equipment

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Image of hands using a pen to complete a form

18th July 2023

SOP: Calibration for Backpack and Truck-Mounted Sprayers

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Ugandan landscape showing a dirt road, trees, house and mountain in the distance

18th July 2023

SOP: Operational Field Trials: Ground and Aerial

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